Abstract
To obtain an overview of how scientists develop new antibodies and find existing ones, the Antibody and Technology Research Group (ARG) used a web-based survey to query academic antibody core facilities/resource centers and researchers. A total of four surveys were conducted using Survey Monkey. The first sought to learn the immunization strategies employed by our peer when they are trying to make antibodies against a weakly immunogenic target covering variable such as host, adjuvant and immunogen form. The second curated screening approaches the cores employ to find hybridomas secreting the desired antibodies, antibodies that bind the target in whatever their final application may be (e.g. flow cytometry, immunoflourescence and in vivo). The third survey focused on the molecular biology side of the field, specifically antibody sequencing and recombinant antibody production. As a follow up to a round table discussion entitled “The Perfect Antibody”, the fourth was conducted to understand how researchers antibodies that meet the needs of their research and if they utilize websites focused on end-user reviews and publication citations.