Fig. 5.
The effect of photon count and extraneous noise on the spacing scenarios. A plot of the strong coupling spacing (Δzsc) and weak coupling spacing (Δzwc) as a function of (a) the photon count (N) and (b) the standard deviation of the readout noise (σ). The ROI size is 32 × 32 pixels. The magnification is 100, the emission wavelength is 520 nm and the numerical aperture is 1.4. In (a), the background level and the standard deviation of the readout noise are 2.5 photons/pixel and 8 e−/pixel, respectively. In (b), the photon count and the background level are 500 photons and 0 photons/pixel, respectively.