Fig. 5.

Tracklet based reconstruction is able to resolve overlapping multi-emitters with improved localization accuracy. (a). Reconstructed image using the SA method. Due to the high density of emitters at the central part of the image, SA is not able to resolve the overlapping emitters, which results in very low number of detected emitters. (b). Tracklet-based reconstruction of the same image. Comparing with (a), the central part of the image is well reconstructed. (c). Image reconstructed with the combined SA method as defined in the main text. (d). Line profiles of the selected regions as shown in (a), (b), and (c). The black curve represents the line profile of the selected region in (A), and the blue curve is from (b), and red is from (c). The blue curve has smaller width, demonstrating the smaller localization uncertainty with the tracklet-based method. Scale bar: 300 nm.