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. 2014 Sep 12;8:109. doi: 10.3389/fncir.2014.00109

FIGURE 2.

FIGURE 2

Development of glycinergic innervation to the SPN of control and Egr2; En1CKO mice. Diffuse GlyT2 staining was present at birth in control (A,A’) and Egr2; En1CKO mice (B,B’). Staining intensity increased by P3 in control (C,C’) and Egr2; En1CKO mice (D,D’). Identifiable boutons were first seen at P7 in control (E,E’) and Egr2; En1CKO (F,F’) mice. Individual boutons are shown with dots and numbers. Staining intensity was also higher in control (G,G’) compared to Egr2; En1CKO mice (H,H’) at P12. However, levels were comparable in control and Egr2; En1CKO mice at P14 (I,I’,J,J’) and adulthood (K,K’,L,L’). (M) GlyT2+ bouton number located on SPN neuronal somata did not change from P7-adulthood in control mice. Bouton numbers were reduced at from P7–P14 in Egr2; En1CKO mice, but were similar to controls in adulthood. Data are represented as mean ± SEM. Higher magnification images of individual neurons are shown in (A’–L’). Scale bars: 20 μm (A–D); 26 μm (E–L); 8 μm (A’–L’). **P < 0.01, ***P < 0.001 vs. age-matched controls and ###P < 0.001 vs. P7 or P12 Egr2; En1CKO mice.