FIGURE 3.

Dendritic localization of GlyT2⁺ boutons is unchanged in adult Egr2; En1^CKO mice. GlyT2+ boutons were present on MAP2+ dendrites of LSO and SPN neurons in adult control (A–A″′,C–C″′) and Egr2; En1^CKO mice (B–B″′,D–D″′). (E) Area outlined by the white box in (A″′). Distance of GlyT2⁺ boutons from neuronal soma was measured as shown. Box plots depicting the median and distribution of bouton distance from cell somata in LSO (F) and SPN (G). Whiskers are representative of minimum and maximum distance values. Data were further analyzed by looking at proximal vs. distal localization by determining the frequency (percentage) of boutons in 5 μm increments for dendrites in the LSO (H) and SPN (I); no significant differences were present between control and Egr2; En1^CKO mice. Scale bars: 33 μm (A–D″′); 16 μm (E).