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. 2014 Aug 22;8:280. doi: 10.3389/fnbeh.2014.00280

Figure 4.

Figure 4

The effect of CART on spontaneous excitatory synaptic transmission in cocaine-treated animals. Traces show 3 s of continuous recording from an aPVT neuron prior to (A, upper), and then during bath application of 10 nmol CART (A, middle), with corresponding amplitude histograms (including baseline noise distributions) below and arrows indicating the mean (cocaine pre-CART, mean amplitude = −18 pA, A, lower left; cocaine post-CART, mean amplitude = −16 pA, A, lower right). Cumulative probability distributions (from representative recordings) showed a significant reduction in both instantaneous frequency (B, upper) and amplitude (B, lower) of aPVT neurons in response to saline or cocaine. Group data plots (C) show sEPSC instantaneous frequency (* p < 0.001), and amplitude (* p < 0.001) were both reduced after CART application. We did not observe a significant effect of CART on rise time, however CART application resulted in a significant increase in decay time constant (* p = 0.11, an average of 1192 events and 669 events were analyzed respectively), with representative traces of rise and decay time pre- and post-CART.