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Examples of epitope and epitope-specific antibodies that we have utilized for studying virus-host protein interactions.
| aEpitope Tag | Mass | Antibody | Ref. | |
|---|---|---|---|---|
| TAP |  |
20 kDa | IgG | (6, 11) |
| GFP |  |
27 kDa | anti-GFP | (1, 2, 5–7, 12–14, 24, 25) |
| FLAG |  |
1 kDa/FLAG | anti-FLAG | (8, 9) |
Epitopes can be introduced at either the amino or carboxyl terminus of the bait protein, or on an internal flexible loop of the protein, provided that it does not interfere with protein function.
