FIG. 3.

Cell viability and transgene expression of amiR vector transduced primary T cells. (A) Eighteen different amiR vectors targeting suppressor of cytokine signaling 1 (SOCS1) were used to transduce primary human T cells along with control vectors and maintained in cell culture for 40 days. Shown is the cell count over time. (B) The same 18 vector-transduced cell cultures were assayed for tCD34' gene expression by FACS at days 10, 30, and 40 post transduction. Shown were the percent CD34 positive cells at the given time points. Columns are labeled to indicate which siRNA was used, the microRNA (miR) backbone, and the vector insertion site. Data shown were from one of two similar experiments using different donors.