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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: FEBS J. 2014 Jul 23;281(16):3545–3558. doi: 10.1111/febs.12898

Figure 3. Effect of suppressing TRX1 on oxidation-reduction of PTEN in cells.

Figure 3

(A) HeLa T-REx-281 (shRNA control) or HeLa T-REx-252 (TRX1 shRNA) cells were treated with 1 µg/ml doxycycline for 7 days. Cells were treated with 500 µM H2O2 and lysate samples were prepared at the time points indicated. Samples were resolved by non-reducing (upper panel) or reducing SDS-PAGE and analyzed by anti-PTEN, anti TRX1 and anti-actin immunoblotting.

(B) Quantitation of a time course of H2O2-induced oxidation of PTEN in the presence (closed circles) and absence (closed squares) of RNAi-mediated suppression of TRX1. The y-axis represents the % of PTEN migrating as the oxidized form on SDS-PAGE compared to the total population of PTEN.