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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: FEBS J. 2014 Jul 23;281(16):3545–3558. doi: 10.1111/febs.12898

Figure 6. Effects of TRX1 on insulin signaling.

Figure 6

293T cells expressing vector only, His6-tagged TRX1-WT or His6-tagged TRX1-C35S constructs were treated with 20 nM insulin for 0, 5, 10, 20 minutes. Insulin-stimulated phosphorylation of the insulin receptor β-subunit was monitored by immunoblotting with anti-insulin receptor [pYpY1162/1163] phospho-specific antibody. Sample loading on the gel was standardized by immunoblotting with antibody to insulin receptor β-subunit.