Table 2. Summary of significantly altered proteins between the LHON cases and the control group.
| Spot ID | Protein name | Gene* | NCBI ID | Identification scores(MS/MS) | %Cov(MS/MS) | number ofmatched peptides(MS/MS) | emPAI | pI | MW (kDa) | Intensity (Mean ± SEM) | Fold changeAffected/Control | P # | FDR | |
| Affected (n = 7) | Control (n = 5) | |||||||||||||
| 242 | Leucine-rich PPRmotif-containingprotein | LRPPRC | gi|31621305 | 1341 | 54 | 72 | 2.22 | 5.81 | 159.00 | 0.0984±0.0104 | 0.1746±0.0225 | 0.48 | 0.008 | 0.009 |
| 295 | Major vaultprotein | MVP | gi|19913410 | 743 | 46 | 41 | 1.68 | 5.34 | 99.55 | 0.0519±0.0043 | 0.0856±0.0062 | 0.61 | 0.001 | 0.003 |
| 301 | Lon proteasehomolog | LONP1 | gi|21396489 | 1087 | 50 | 50 | 2.53 | 6.01 | 106.94 | 0.0414±0.0054 | 0.0861±0.0063 | 0.48 | <0.001 | <0.003 |
| 306 | Vinculin isoformVCL | VCL | gi|4507877 | 1361 | 59 | 64 | 4.01 | 5.83 | 117.22 | 0.1089±0.0068 | 0.1704±0.0182 | 0.64 | 0.005 | 0.007 |
| 388 | Transmembraneprotein – mitofilin | IMMT | gi|1160963 | 1174 | 67 | 57 | 5.97 | 6.15 | 83.89 | 0.1586±0.0121 | 0.2506±0.0168 | 0.63 | 0.001 | 0.003 |
| 396 | Methylmalonyl-CoA mutase | MUT | gi|187452 | 522 | 34 | 21 | 1 | 6.48 | 83.54 | 0.0516±0.0051 | 0.0843±0.0069 | 0.61 | 0.012 | 0.010 |
| 424 | Trifunctionalenzyme subunitalpha | HADHA | gi|20127408 | 1297 | 65 | 55 | 4.41 | 9.16 | 83.69 | 0.6631±0.0725 | 1.0434±0.1170 | 0.64 | 0.048 | 0.0232 |
| 448 | MTHSP75 | HSPA9 | gi|292059 | 2174 | 68 | 84 | 11.1 | 5.97 | 74.02 | 0.4530±0.0536 | 0.7416±0.1002 | 0.61 | 0.030 | 0.017 |
| 469 | Cellular myosinheavy chain | MYH9 | gi|553596 | 604 | 24 | 25 | 0.6 | 5.70 | 155.29 | 0.0815±0.0054 | 0.1414±0.0260 | 0.58 | 0.041 | 0.021 |
| 473 | Glycerol-3-phosphatedehydrogenase | GPD2 | gi|1020315 | 1080 | 64 | 47 | 3.98 | 7.58 | 81.30 | 0.0341±0.0052 | 0.0660±0.0100 | 0.52 | 0.018 | 0.013 |
| 490 | Succinate dehydrogenase[ubiquinone] flavoproteinsubunit | SDHA | gi|156416003 | 1019 | 57 | 39 | 2.96 | 7.06 | 73.67 | 0.1323±0.0105 | 0.2242±0.0338 | 0.59 | 0.018 | 0.013 |
| 512 | Very long-chainspecific acyl-CoAdehydrogenase | ACADVL | gi|4557235 | 963 | 64 | 38 | 4.14 | 8.92 | 70.35 | 0.0898±0.0129 | 0.1423±0.0096 | 0.63 | 0.018 | 0.013 |
| 551 | 60 kDa heatshock protein | HSPD1 | gi|31542947 | 2360 | 77 | 99 | 14.12 | 5.7 | 61.19 | 0.6804±0.5417 | 0.9936±0.0941 | 0.68 | 0.037 | 0.020 |
| 556 | Catalase | CAT | gi|4557014 | 694 | 55 | 25 | 2.54 | 6.9 | 59.95 | 0.0648±0.0095 | 0.1004±0.0062 | 0.65 | 0.025 | 0.015 |
| 587 | Dihydrolipoamide DehydrogenaseAnd DihydrolipoamideDehydrogenase-BindingProtein (Didomain) SubcomplexOf Human PyruvateDehydrogenase Complex. | DLD | gi|83753870 | 563 | 51 | 28 | 2.86 | 7.95 | 54.18 | 0.1873±0.0124 | 0.2402±0.0184 | 0.78 | 0.047 | 0.023 |
| 862 | Pyruvate dehydrogenase E1 component subunit alpha | PDHA1 | gi|33357460 | 572 | 36 | 17 | 1.87 | 8.35 | 43.30 | 0.6067±0.0425 | 0.7545±0.0185 | 0.80 | 0.039 | 0.020 |
| 935 | Electron transfer flavoprotein subunit alpha, mitochondrial isoform a | ETFA | gi|4503607 | 343 | 63 | 17 | 3.47 | 8.62 | 33.42 | 0.2686±0.0210 | 0.3711±0.0162 | 0.72 | 0.007 | 0.008 |
*Gene name according to HUGO Gene Nomenclature Committee.
#
indicates P value based on Post Hoc Tukey Test after one way ANOVA and the value less than 0.05 is considered significant in both tests.