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. 2014 Sep 12;9(9):e106913. doi: 10.1371/journal.pone.0106913

Figure 4. Lipid-reduced (LR) medium conditions increase the activation of SREBP1 and SREBP2 in some but not all cancer cell lines.

Figure 4

(a) Representative virtual blot of Simple Western analysis of precursor and active SREBP1 expression in HepG2, PC3M, HOP62 and T24 cells after 72 hours cultivation in normal (N) or LR medium conditions. Alpha-tubulin is used as a loading control. Different exposures of precursor and active SREBP1 are shown in order to have an accurate exposure for both forms. For original data see Supplementary Figure S3a-d. (b) Quantitative analysis of Simple Western. data, expressed as area under the curve (AUC). Expression of SREBP1 was corrected for the loading control alpha-tubulin. Graph represents mean ± S.D. (n = 2–3). (c) Representative virtual blot of Simple Western analysis of precursor and active SREBP2 expression in HepG2, PC3M, HOP62 and T24 cells after 72 hours cultivation in normal (N) or LR medium conditions. Alpha-tubulin is used as a loading control. Different exposures of precursor and active SREBP2 are shown in order to have an accurate exposure for both forms. For original data see Supplementary Figure S3e-h. (d) Quantificative analysis of Simple Western. data, expressed as area under the curve (AUC). Expression of SREBP2 was corrected for the loading control alpha-tubulin. Graph represents mean ± S.D. (n = 2–3).