Table 2. PCR Primer pairs for amplification of the TYR gene in OCA1 patients.

Gene name	Primer name	Exon	Locus (nDNA)	Primer sequence (5′ to 3′)	Amplicon size (bp)	Temperature (°C)
TYR	AL-1-1F	1–1*	88910556	CAAACTGAAATTCAATAACATATAAGG	678	59
TYR	AL-1-1R		88911233	GTGGACAGCATTCCTTCTCC
TYR	AL-1-2F	1–2*	88910948	TTCAGAGGATGAAAGCTTAAGATAAA	521	59
TYR	AL-1-2R		88911468	CGTCTCTCTGTGCAGTTTGG
TYR	AL-1-3F	1–3*	88911171	CTGGCCATTTCCCTAGAGC	605	60
TYR	AL-1-3R		88911775	CCACCGCAACAAGAAGAGTC
TYR	AL-1-4F	1–4*	88911487	CATCTTCGATTTGAGTGCCC	521	59
TYR	AL-1-4R		88912007	CCCTGCCTGAAGAAGTGATT
TYR	AL-2F	2	88924298	CCAACATTTCTGCCTTCTCC	442	60
TYR	AL-2R		88924739	TCAGCTAGGGTCATTGTCGAT
TYR	AL-3F	3	88960909	AGTTATAAATCAAATGGGATAATCA	296	56
TYR	AL-3R		88961204	ACATTTGATAGGCACCCTCT
TYR	AL-4F	4	89017552	CTGTTTCCAATTTAGTTTTATAC	790	56
TYR	AL-4R		89018341	TACAAAATGGCCTATGTTAAGC
TYR	AL-5F	5	89028218	TGTCTACTCCAAAGGACTGT	924	55
TYR	AL-5R		89029138	GGCACTTAGCTGGATGTGTT

*Shows that due to the large size of exon 1, it is divided into four overlapping fragments.