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. 2014 Sep 12;9(9):e107505. doi: 10.1371/journal.pone.0107505

Figure 7. PP4-deficient B cells exhibit increased etoposide-induced caspase 3 cleavage.

Figure 7

(A) FACS profiles of AnnexinV vs 7AAD staining of WT and CD23crePP4F/F B cells that were cultured for 6 h (not shown) or 24 h in maintenance medium alone or in maintenance medium containing 1 µM etoposide. (B) Quantitation of the percentage of viable (AnnexinV−7AAD−) cells among total B cells from the data in (A). Data are the mean ± SD of three mice per group. For (A–B), results are representative of two independent experiments. (C) FACS profiles of B220 vs cleaved caspase 3 staining of WT and CD23crePP4F/F B cells in the resting state (Resting), cultured in maintenance medium alone (Medium) or in maintenance medium containing 1 µM etoposide for 10 h or 15 h. (D) Quantitation of the percentage of B cells exhibiting cleaved caspase 3 among total B cells from data in (C). Data are the mean ± SD of three mice per group. For (C–D), results are representative of two independent experiments.