Figure 4. Schematic representation of a single cross-over event between plasmid pMG601 and H. salinarum R1.

A and B, illustration of WT and the mutant genotype after incorporation of plasmid pMG501, respectively, C, Agarose gel of PCR products from chromosomal DNA of strain R1 (lane 2), and chromosomal DNA from 3 BgaH⁺ colonies after integration of pMG601 into R1 (lane 3–5), MW marker is shown in lane 1 (in bp).