Figure 3. An example of the VfoldThermal prediction: the inputs highlighted in the snapshot of VfoldThermal web server are the sequence (32 nts in this example) with the temperature range of 0°C–100°C, the energy parameters used for base stacks (from MFOLD in this example) and the structure type (non-pseudoknotted in this example).

From the temperature dependence of the partition function Q(T), VfoldThermal gives a list of temperature-dependent heat capacity C(T), with temperature interval of 0.5°C. The eps format of melting curve is generated by Gnuplot.