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. 2014 Sep 12;9(9):e107265. doi: 10.1371/journal.pone.0107265

Figure 2. Clodronate liposome-mediated KC and VATM depletion ameliorates hepatic steatosis in DIO mice.

Figure 2

Wild type mice were fed a HFD for 13 weeks and injected with 2 doses of clodronate liposomes (250 mg/kg) or PBS liposomes by i.p. over 6 days with a 3-day interval. A.) Livers of DIO mice were isolated, fixed in 10% formalin, embedded in paraffin, and stained with H&E or frozen in OCT and stained with Oil-Red-O to assess steatosis. Images are representative of 12–16 animals at 10X and 20X magnification. B.) Livers of DIO animals were weighed and data is represented as a percentage of total body weight. C.) Total TGs were extracted and normalized to tissue weight. Values represent the mean ± SEM. An unpaired student’s t-test was used for comparisons between groups. *P≤0.05, **P≤0.01.