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. 2014 Sep 12;9(9):e107265. doi: 10.1371/journal.pone.0107265

Figure 3. Clodronate-encapsulated liposomes deplete KCs but not VATMs in ob/ob mice.

Figure 3

8-week ob/ob mice were injected with 2 doses of clodronate liposomes (250 mg/kg) or PBS liposomes by i.p. for 6 days with a 3-day interval. We observed no changes in the total animal body weight of PBS liposome-treated mice (44.2±0.7 to 45.2±0.6) or clodronate -treated mice (41.9±1.4 to 41.1±1.7). VAT SVF was isolated and FACS analysis was performed. A.) Upper panels, PBS liposome-treated mice. Lower panels, clodronate-treated animals. Left panels, SiglecF-negative, CD11b- and F4/80-positive cells. Right panels, CD11c-positive cells. Panels are representative of 4–6 animals per group. B.) Percentage of the total number of cells counted for both CD11b- and F4/80-positive cells and CD11c-positive cells in the VAT of ob/ob mice. RNA was extracted and quantitative RT-PCR was performed for standard macrophage markers (F480, CD11c) in C.) VAT and D.) liver of ob/ob mice. Values represent the mean ± SEM. An unpaired student’s t-test was used for comparisons between groups. *P≤0.05, ***P≤0.001.