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. 2014 Sep 12;9(9):e107265. doi: 10.1371/journal.pone.0107265

Figure 4. Clodronate liposome-mediated KC depletion ameliorates hepatic steatosis in ob/ob mice.

Figure 4

8-week old male ob/ob mice were injected with 2 doses of clodronate liposomes (250 mg/kg) or PBS liposomes by i.p. over 6 days with a 3-day interval. A.) Livers of ob/ob animals were isolated, fixed in 10% formalin, embedded in paraffin, and stained with H&E or frozen in OCT and stained with Oil-Red-O to assess steatosis. Images are representative of 7–10 animals at 10X and 20X magnification. B.) Livers of ob/ob animals were weighed and data is represented as a percentage of total body weight. C.) Total TGs were extracted and normalized to tissue weight. Values represent the mean ± SEM. An unpaired student’s t-test was used for comparisons between groups. *P≤0.05, ***P≤0.001.