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. 2014 Sep 12;9(9):e107517. doi: 10.1371/journal.pone.0107517

Figure 4. Differentiation of NSCs in vitro.

Figure 4

(A–I) Cells cultured on PCLA (A, D, G), PCLA-SF (B, E, H), and PCLA-SF-NT-3 (C, F, I) membrane were immunostained with MAP-2 for neurons (A–C), GFPA for astrocytes (D–F), CNPase for oligodendrocytes (G–I). The nuclei were stained with DAPI. (J) Quantitative analysis of the NSC differentiation in vitro. Scale bars = 25 µm, *P<0.05; n = 4.