Figure 1. Time- and concentration-dependent inactivation of elementary bodies (EBs) of GFP-L2, derived from C. trachomatis serovar L2, by Lactobacillus-conditioned medium (LCM).

LCM was collected from overnight still cultures of L. crispatus 33197 (Lc33197), L. crispatus 33820 (Lc33820), L. gasseri 33323 (Lg33323) and L. jensenii 25258 (Lj25258). pH values of undiluted (100%) LCM are shown. EBs were treated with undiluted LCM or LCM diluted 10 fold with 0.9% NaCl for 5 min or 1 h. Following treatments, surviving EBs were serially diluted and then inoculated onto McCoy monolayers; resulting inclusion-forming units were scored by fluorescence microscopy. Values were averages ± standard deviations of triplicate experiments.