Figure 3. Complete reversal of chlamydiacidal activity with pH-neutralized LCM.
LCM from overnight still cultures of Lactobacillus strains or control MRS broth was adjusted to pH 7.0. GFP-L2 EBs were treated with pH-unadjusted LCM (pH value shown for each on the horizontal X-axis) or neutralized LCM for 1 h. Surviving bacteria were quantified as outlined in Fig. 1 legend. Values were averages ± standard deviations of triplicate experiments. Double asterisks above LCM-treated samples denote highly statistically increased IFUs (P<0.01) as a result in treatment with neutralized LCM as compared to pH-unadjusted LCM.