Figure 3. In vitro binding of Rex to ldhA promoter region determined by EMSA.

Panel A. Biotin-labeled PCR products (2 nM) corresponding to ldhA and rex promoter regions and a negative control (a fragment of ssuRNA BC_0007 sequence) were mixed with increasing concentrations of purified Rex. Panel B. PCR product corresponding to the ldhA promoter region was incubated with 0.6 µM purified Rex and different concentrations of NAD⁺ and NADH, as indicated.