Table 1.
PCR primers used for generating analytical or preparative products from genomic and genomic DNA-derived PCR templates are indicated
| Primer | Sequence (5′–3′) | Product (bp) |
|---|---|---|
| CF1 (f) CF5 (r) |
GCAGAGTACCTGAAACAGGA CATTCACAGTAGCTTACCCA |
491 (wt)/488 (delF508) |
| CF1B (f) | CCTTCTCTGTGAACCTCTATCA | |
| CF7C (r) | ATAGGAAACACCAAAGATGA | 392 |
| CF8C (r) | ATAGGAAACACCAATGATAT | 389 |
| CF17 (f) | GAGGGATTTGGGGAATTATTTG | |
| CF7C (r) | ATAGGAAACACCAAAGATGA | 330 |
| CF8C (r) | ATAGGAAACACCAATGATAT | 327 |
Primer pair CF1/CF5 was used to generate preparative quantities of SDF by PCR. Analysis of the wtCFTR and delF508-CFTR alleles in genomic DNA was performed by AS-PCR with primer pairs CF1B/CF7C (wt) and CF1B/CF8C (delF508). Primers CF7C and CF8C are allele specific and common to both DNA and RNA analyses; however, when paired with the non-allele-specific primer CF17 in exon10, amplification is specific for RNA-derived cDNA (AS-RT-PCR) crosses intron/exon boundaries. Forward primer = f, Reverse primer = r