Figure S4.

Comparative Expression Analysis, Related to Figure 5

(A) Genes contributing to principal components distinguishing reset cells from conventional human PSC. Gene symbols were extracted from the PCA and the labels scaled relative to the magnitude of variance. Pluripotency regulators are present in the leftmost area defining reset cells, whereas numerous lineage-specific genes can be found to the right expressed in conventional human PSC cultures.

(B) Platform-specific principal component analysis of RNA-seq data. Data are compared from reset cells, mouse ESC cultured in three different conditions, conventional human PSC and 3iL samples from Chan et al. Clustering of cell types when applied to a single technology recapitulates the integrated analysis in Figure 5A.