Figure S9.

Knockdown of aPKC iota/lambda and zeta, Related to Discussion

Two different shRNA vectors, shPKC iota_1 and shPKC iota_4, were used for knockdown of PKC iota/lambda. shPKCzeta_8 was used for knockdown of PKC zeta. Scale bars: 100 μM.

(A) Brightfield images of shPKC iota KD and shPKC zeta KD cells cultured in t2iL. shPKC iota KD cell retain undifferentiated morphology whereas shPKC zeta cells progressively differentiate.

(B) Knockdown efficiency of each shRNA.

(C) OCT4 expression at passage 10 (KD lines) or 3 (control).

Error bars indicate SD.