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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: Nature. 2014 Jul 20;513(7517):251–255. doi: 10.1038/nature13557

Extended Data Figure 8. FBP1 regulates glucose metabolism and HIF activity in a catalytic activity-independent manner.

Extended Data Figure 8

a, Protein levels of ectopically expressed V5-FBP1 and V5-FBP1 G260R mutant in 293T and RCC10 cells. Actin was used as a loading control. b, FBP1 enzymatic activity in the 293T cell lysates as shown in (a). c, Western blot analysis of V5-tagged proteins and actin in 786-O and RCC10VHL cells ectopically expressing vector, V5-FBP1, and V5-FBP1 G260R. d, Growth of 786-O cells expressing vector, FBP1, or FBP1 G260R in 1% serum medium. Glucose uptake (e), lactate secretion (f), relative NADPH levels (g), and indicated HIF target gene expression (h) in RCC10 cells expressing vector, FBP1, or FBP1 G260R. i, Growth of RCC10VHL cells expressing vector, FBP1, or FBP1 G260R in 1% serum medium. Glucose uptake (j), lactate secretion (k), relative NADPH levels (l), and indicated HIF target gene expression (m) in RCC10VHL cells expressing vector, FBP1, or FBP1 G260R. Experiments were repeated twice. Values represent mean±s.d. (technical triplicates from a representative experiment) *p<0.01.