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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: Nature. 2014 Jul 20;513(7517):251–255. doi: 10.1038/nature13557

Figure 2. FBP1 regulates glycolysis and NADPH levels.

Figure 2

a, Glucose uptake and lactate secretion in HK-2 cells with or without FBP1 inhibition. M2 isotopomer distribution of indicated metabolites (b) and citrate (c) in HK-2 cells with or without FBP1 ablation, labelled with [1, 2-13C] glucose. % M2 enrichment represents the mole percent excess of M2 species above natural abundance. d, Glucose uptake and lactate secretion in RCC10 and RCC10VHL cells ectopically expressing vector or FBP1. RCC10VHL cells are RCC10 cells where wild-type pVHL has been reintroduced. e, M2 isotopomer distribution of indicated metabolites in RCC10 cells expressing vector or FBP1, labelled with [1, 2-13C] glucose. f, Relative NADPH levels in RCC10 and RCC10VHL cells as indicated in (d). Values represent mean±s.d. (three experimental replicates). *p<0.05.