Fig. 5.

(A and B) Representative images of immunohistochemical analysis of VSELs isolated by fluorescence-activated cell sorting from bone marrow and peripheral blood after KA administration (25 mg/kg b.w). (A) BM-derived Sca1⁺/Lin⁻/CD45⁻ cells express Oct-4 and nestin. (B) PB-derived Sca1⁺/Lin⁻/CD45⁻ cells express Oct-4 and nestin. All images were taken under a Plan Apo 60XA/1.40 oil objective (Nikon). Staining was performed on cells isolated from three independent sorts with similar results. (C) RT-PCR analysis of the expression of selected pluripotency genes: Oct4, Nanog, and the neural marker nestin in cells sorted from bone marrow of control and kainic acid-treated mice (25 mg/kg b.w). Lane 1, control mouse; Lane 2, kainic acid-treated mouse; Lane 3, H₂O negative control. GAPDH expression served as loading control. A representative analysis is shown.