Table D1.
Typical parameter values for E. coli growth.
| Source | Strain | Experimental conditions | Parameter | Value |
|---|---|---|---|---|
| Bernstein et al. (2012) | E. coli MG1655 WT | Aerobic batch growth, 37 °C, M9 minimal media, acetate consumption deletion | Biomass yield (CDW[g]/g of glucose) | 0.23 |
| mutant 307 | Maximum growth rate on 4 g/L glucose (h−1) | 0.43–0.49a | ||
| mutant 403 | glucose consumption deletion | Maximum growth rate on 1.5 g/L acetate (h−1) | 0.11–0.15a | |
| Chen et al. (1997) | E. coli K-12 MG1655 | Aerobic batch growth, complex medium, 37 °C | Maximum growth rate on glucose (h−1) | 0.57 |
| Biomass yield (CDW[g]/g of glucose) | 0.255 | |||
| Chen et al. (1997) | E. coli K-12 MG1655 | Anaerobic batch growth, complex medium, 37 °C | Maximum growth rate on glucose (h−1) | 0.43 |
| Biomass yield (CDW[g]/g of glucose) | 0.175 | |||
| Dykhuizen (1978) | E. coli K-12 W3110 | Aerobic batch growth, Davis salts, 37 °C | Maximum growth rate on glucose (h−1) | 0.76 |
| Dykhuizen (1978) | E. coli K-12 W3110 | Chemostat growth, Davis salts, 37 °C | Glucose half-saturation constant (mg/L) | 7.2 |
| Edwards et al. (2001) | E. coli K-12 MG1655 | Aerobic batch growth, M9 minimal media 27.5–37 °C, 0.05–4 g/L acetate | Maximum growth rate on acetate (h−1) | 0.31 |
| Biomass yield (CDW[g]/g of acetate) | 0.38 | |||
| Farmer and Liao (1997) | E coli K-12 VJS632 | Aerobic batch growth, 37 °C, M9 minimal media, 0.5% glucose | Maximum growth rate on glucose(h−1) | 0.52 |
| Biomass yield (CDW[g]/g of glucose) | 0.30 | |||
| Acetate yield (g of acetate/g of glucose) | 0.25 | |||
| Review paper | E. coli various | various | Glucose half-saturation constant (mg/L) | 0.04–180 |
| Fuhrer et al. (2005) | E. coli K-12 MG1655 | Aerobic batch growth, 30 °C, M9 minimal media, 3 g/L glucose | Maximum growth rate on glucose (h−1) | 0.39 |
| Biomass yield (CDW[g]/g of glucose) | 0.30 | |||
| Guardia and Calvo (2001) | E. coli ATCC 8789 | Aerobic batch and chemostat growth, 37 °C, complex medium including 5 g/L yeast extract, varying 1–19 g/L glucose, parameter fits to model | Maximum growth rate on glucose (h−1) | 1.12 |
| Glucose half-saturation constant (mg/L) | 100 | |||
| Acetate product inhibition constant (g/L) | 4 | |||
| Maximum growth rate on acetate (h−1) | 0.19 | |||
| Acetate half-saturation constant (g/L) | 0.4 | |||
| Acetate substrate inhibition constant (g/L) | 3.8 | |||
| Acetate yield (g acetate produced/CDW[g]) | 0.4 | |||
| Kleman and Strohl (1994) | E. coli K-12 W3110 | Aerobic fed-batch with target 0.5 g/L glucose, mineral medium, 35 °C | Maximum growth rate on glucose (h−1) | 0.91 |
| Glucose half-saturation constant (mg/L) | 4 | |||
| Biomass yield (CDW[g]/g of glucose) | 0.43 | |||
| Acetate yield (g of acetate/g of glucose) | 0.38 | |||
| Acetate yield (g acetate produced/CDW[g]) | 0.3–0.9 | |||
| Natarajan and Srienc (2000) | E. coli BL21 | Chemostat, 30 °C, dilution rate 0.12–0.40 h−1 inflow: M9 minimal media, 0.25 g/L glucose | Maximum growth rate on glucose (h−1) | 0.768 |
| Glucose half-saturation constant (mg/L) | 0.68 | |||
| Oh et al. (2002) | E. coli MC4100 | Aerobic batch growth, M9 minimal media | Maximum growth rate on 0.25% acetate (h−1) | 0.33 |
| Paalme et al. (1997) | E. coli K-12 W3350 | A-stat, 37 °C, mineral medium, 10 g/L glucose | Maximum growth rate on glucose (h−1) | 0.57 |
| Biomass yield (CDW[g]/g of glucose) | 0.50 | |||
| Paalme et al. (1997) | E. coli K-12 W3350 | A-stat, 37 °C, mineral medium, 5 g/L acetate | Maximum growth rate on acetate (h−1) | 0.22 |
| Biomass yield (CDW[g]/g of acetate) max/min | 0.30/0.21 | |||
| Senn et al. (1994) | E. coli ML30 | Chemostat growth, mineral medium, 37 °C | Maximum growth rate on glucose (h−1) | 0.92 |
| Glucose half-saturation constant (mg/L) | 0.0072 | |||
| Literature review Senn et al. (1994) | E. coli various | various | Maximum growth rate on glucose (h−1) | 0.55–1.23 |
| Glucose half-saturation constant (mg/L) | 0.0053–99 | |||
| Varma and Palsson (1994) | E. coli K-12 W3110 | Aerobic batch growth, 38 °C, M9 minimal media, 2 g/L glucose | Maximum growth rate on glucose (h−1) | 0.68 |
| Biomass yield (CDW[g]/g of glucose) | 0.36 | |||
| Varma and Palsson (1994) | E. coli K-12 W3110 | Anaerobic batch growth, 38 °C, M9 minimal media, 2 g/L glucose | Maximum growth rate on glucose (h−1) | 0.43 |
| Biomass yield (CDW[g]/g of glucose) | 0.13 | |||
| Xu et al. (1999) | E. coli K-12 W3110 | Aerobic batch growth, 35 °C, mineral medium, 4–14 g/L glucose | Maximum growth rate on glucose (h−1) | 0.55 |
| Biomass yield (CDW[g]/g of glucose) | 0.52 | |||
| Maximum growth rate on acetate (h−1) | 0.05 | |||
| Xu et al. (1999) | E. coli K-12 W3110 | Aerobic batch growth, 35 °C, mineral medium, 5 g/L glucose, 0–8 g/L acetate | Acetate product inhibition constant (g/L) | 9 |
| Biomass yield (CDW[g]/g of acetate) | 0.4 | |||
| Xu et al. (1999) | E. coli K-12 W3110 | Aerobic fed-batch (exponential feeding rate) with target μ = 0:3 h−1, mineral medium, 35 °C | Maximum growth rate on glucose (h−1) | 0.54 |
| Glucose half-saturation constant (mg/L) | 50 | |||
| Acetate product inhibition constant (g/L) | 4, 5 | |||
| Aerobic biomass yield (CDW[g]/g of glucose) | 0.49, 0.51 | |||
| Anaerobic biomass yield (CDW[g]/g of glucose) | 0.15 | |||
| Maximum growth rate on acetate (h−1) | 0.06, 0.08 | |||
| Acetate half-saturation constant (g/L) | 0.05 | |||
| Biomass yield (CDW[g]/g of acetate) | 0.4 |
Note: When parameters were from the fit to a specific inhibition model, the parameters reported in are the ‘effective’ parameters, i.e., the reported ‘inhibition constants’ are the concentration at which growth rate has decreased to half its maximum. Similarly, the ‘half-saturation constants’ are the concentrations at which growth has increased to half its maximum, and the ‘maximum growth rates’ are the maximum possible growth rates.
a
(before - after) adaptation to a single carbon source.