Table 3.
Predicted antigenic sites of 23 pest and 8 plant β-CA primary sequences
| Species name | Entry ID | β-CA ID | Pest or plant | HitCount * | The most antigenic epitope |
|---|---|---|---|---|---|
| Acyrthosiphon pisum | J9K706 | BCA1 | Pest | 14 | 77 YTSCEPAALELGCVHNDIRHVIVCGHSDC 105 |
| Acyrthosiphon pisum | Predicted | BCA2 | Pest | 14 | 79 TCEPAALELGCVHNDIRHVIVCGHSDC 105 |
| Ancylostoma caninum | FC551456 | BCA | Pest | 11 | 101 INHVIVCGHSDCKAINTLYNIHECPHTFDP 130 |
| Ascaris suum | F1LE18 | BCA | Pest | 15 | 102 KHAIVCGHSDCKAMST 117 |
| Caligus clemensi | C1C2M7 | BCA | Pest | 10 | 84 EPAGLELGCVLNSIKNVIVCGHSDCKAMIAVHSL 117 |
| Camponotus floridanus | E2ANQ9 | BCA | Pest | 11 | 80 CESAALELGCVVNDIRHVIVCGHSDC 105 |
| Ceratitis capitata | XP_004537221.1 | BCA | Pest | 13 | 72 HFQDEYFSCEPAALELGCVINDIRHIIVCGHSD 104 |
| Culex quinquefasciatus | B0WKV7 | BCA | Pest | 14 | 75 DEYFSCEPAALELGCVVNNIKHIIVCGHSDC 105 |
| Dendroctonus ponderosae | J3JTM9 | BCA | Pest | 13 | 95 RHIIVCGHSDCKAINLLYKL 114 |
| Entamoeba dispar | B0E7M0 | BCA | Pest | 8 | 85 SIEYGVTHLKTPLIVVLSHTSCGACTAACQRA 116 |
| Entamoeba histolytica | C4LXK3 | BCA | Pest | 8 | 83 LGSVEYGVTHLKTPLIVVLSHTSCGACTAACQRA 116 |
| Entamoeba nuttalli | K2GQM0 | BCA | Pest | 7 | 83 LGSVEYGVTHLKTPLIVVLSHTSCGACTAACKHA 116 |
| Haemonchus contortus | U6PDI1 | BCA | Pest | 13 | 101 HINHVIVCGHADCKAINTLYNL 122 |
| Ichthyophthirius multifiliis | G0QYZ1 | BCA1 | Pest | 13 | 193 ANQVIHTDLNCLSVVQYAVEVLKVSDIIICGHYKCGGVHAAVKNT 237 |
| Ichthyophthirius multifiliis | G0QPN9 | BCA2 | Pest | 9 | 86 ANQVIHTDLNCLSVIQYAVDVLNIKDIIVCGHYECGGVAASIANPKLGL 134 |
| Ichthyophthirius multifiliis | Predicted | BCA3 | Pest | 7 | 65 ANQVIHTDLNCLSVVQFAVEVLKVTDIIICGHYKCGGVNAA 105 |
| Ichthyophthirius multifiliis | Predicted | BCA4 | Pest | 6 | 62 ANQVIHTDLNCLSVVQFAVEVLKVTDIIICGHYKCGGVNA 101 |
| Lepeophtheirus salmonis | D3PI48 | BCA | Pest | 10 | 82 PEPAGLELGCVVNSIKNVVVCGHSDCKAMIALQSF 116 |
| Necator americanus | ETN68732.1 | BCA | Pest | 10 | 108 HINHVIVCGHSDCKAINTLYNIHTCPQ 134 |
| Solenopsis invicta | E9IP13 | BCA | Pest | 14 | 97 CESAALELGCVVNDIKHVIVCGHSDC 122 |
| Tribolium castaneum | D6WK56 | BCA | Pest | 13 | 116 ALELGCVVNDIRHIIVCGHSDCKAINLLYKLQDS 149 |
| Trichinella spiralis | E5SH53 | BCA | Pest | 11 | 100 KDIVVCGHSDC 110 |
| Trichoplax adhaerens | B3S5Y1 | BCA | Pest | 13 | 82 EAAALELACVRNQVSSVVVCGHSDC 106 |
| Arabidopsis thaliana | Q9ZUC2 | BCA | Plant | 13 | 80 PKFLVFACADSRVSPSHILNFQ 101 |
| Pisum sativum | P17067 | BCA | Plant | 16 | 153 PFMVFACSDSRVCPSHVLDFQ 173 |
| Gossypium hirsutum | Q8LSC8 | BCA | Plant | 14 | 151 KYMIVACSDSRVCPSHVLDM 170 |
| Nicotiana tabacum | P27141 | BCA | Plant | 15 | 146 KFMVFACSDSRVCPSHVLNF 165 |
| Vitis vinifera | D7TWP2 | BCA | Plant | 13 | 149 KFMVFACSDSRVCPSHVLDFQ 169 |
| Solanum tuberosum | I2FJZ8 | BCA | Plant | 15 | 146 KFMVFACSDSRVCPSHVLNF 165 |
| Populus trichocarpa | B9GHR1 | BCA | Plant | 13 | 146 KFMVFACSDSRVCPSHVLDFQ 166 |
| Capsella rubella | R0H8X7 | BCA | Plant | 11 | 84 KYMVFACSDSRVCPSHILNFH 104 |
The italic and bolded residues represent the first (CXDXR) and second (HXXC) highly conserved sequences in the catalytic active sites of the enzyme whenever present in the predicted epitope.
*:HitCount means the total number of antigenic residues in the whole sequence of one protein or antigen.