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. 2013 Dec 19;9(4):474–482. doi: 10.1002/biot.201300336

Figure 1.

Figure 1

Compositions of the reaction mixtures used in the microtiter plate assays shown in Fig. 3, each well contained additionally 450 μM DCPIP, 75 μM MG, or 100 μM Thi as indicated. These dye concentrations were selected so that changes in the absorption can be detected by eye, which is not possible when higher concentrations are used. The first column represents the negative control (no enzyme added). Line A: POx, pyranose 2-oxidase from Trametes multicolor; B: PDH, pyranose dehydrogenase from Agaricus meleagris; C: CDH, cellobiose dehydrogenase from Neurospora crassa; D: DAAO, D-amino acid oxidase from Trigonopsis variabilis; E: LOx, L-lactate oxidase from Aerococcus viridans. PPB, phosphate buffer; d-Gluc, d-glucose; d-MET, d-methionine; LLA, l-lactic acid.