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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cell. 2014 Sep 11;158(6):1375–1388. doi: 10.1016/j.cell.2014.07.050

Figure 3. Recruitment of Cdc48p to Ubiquitinated Hrd1p.

Figure 3

(A) Bead-immobilized Hrd1p (1 μM) was ubiquitinated and incubated at different salt conditions with 2 μM of the indicated components (UN, Ufd1p/Npl4p) in the presence of 250 μM ATPγS. The bound material was analyzed by SDS-PAGE and staining with IRDye Blue.

(B) As in (A), but bead-immobilized Hrd1p was modified with methylated ubiquitin.

(C) As in (A), but with non-ubiquitinated Hrd1p.

See also Figure S3.