Figure 1. Experimental design for analysis of cell of origin.
The inducible CK5-CreERT2 driver can lineage-mark basal cells by YFP expression in different prostate cancer models prior to overt cancer formation. Similarly, the inducible PSA-CreERT2 and CK8-CreERT2 drivers can mark luminal cells in phenotypically normal epithelium. The presence of YFP+ cell clusters in subsequent PIN/cancer lesions indicates that the marked cell type acts as the cell of origin in the mouse model analyzed.