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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2014 Aug 19;25(10):1675–1693. doi: 10.1007/s13361-014-0965-1

Figure 4.

Figure 4

Supercharging agents increase charge monotonically as more and more reagent is added, whereas the onset of denaturation is marked by the sharp onset of a second distribution. ESI-MS of equine holo-myoglobin with (a) 0%, (b) 0.5% (c) 2%, and (d) 5% propylene carbonate (v/v) show smoothly increasing CSDs.