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. Author manuscript; available in PMC: 2015 Nov 24.
Published in final edited form as: Life Sci. 2014 Mar 13;118(2):195–199. doi: 10.1016/j.lfs.2014.03.003

Figure 3. Effect of anti-miR inhibitors on target mRNA levels.

Figure 3

Levels of mRNAs were determined by qPCR. Probes for qPCR were as follows: beta actin, ACTGAGCTGCGTTTTACACCCTTTC; Edn1, ACTGAGCTGCGTTTTACACCCTTTC; Sgk1, CTCTACGGCCTGCCCCCGTTTTATA, Scn2a1, GATCTTTCCGACTGCTTAGAGTCTT; Atp1b1, GCCCCGCCAGGATTGACACAGATTC. Panels: A, mIMCD-3 cells were transfected with anti-miR-709 for 48 hours; B, anti-miR-1898 for 48 hours; C, anti- 135a-3p for 48 hours. mRNA expression levels were normalized to a β actin control. *, p < 0.05, ‡, p < 0.08, **, p < 0.01