TABLE 4.
Candidate miRNAs possibly targeting the murine Edn1 3’ UTR
| miRNA | Microarraya | ||||
|---|---|---|---|---|---|
| Highly expressed | Aldosterone | Vehicle | Total Context Scoreb | miRANDAc | Sitesd |
| miR-709 | 10.26 ± .14 | 10.52 ± .60 | −0.31 | −0.52 | 212^,610,668 |
| let-7a | 9.50 ± .38 | 9.32 ± .54 | −0.34 | −0.99 | 563 |
| let-7c | 8.95 ± .13 | 8.97 ± .52 | −0.34 | −0.99 | 563 |
| let-7d | 8.83 ± .26 | 8.83 ± .38 | −0.34 | −1.01 | 563 |
| let-7f | 8.67 ± .38 | 8.20 ±1.11 | −0.34 | −0.99 | 563 |
| let-7b | 8.95 ± .17 | 8.27 ± .35 | −0.34 | −0.99 | 563 |
| miR-31 | 7.65 ± .22 | 7.42 ± .20 | −0.20 | −1.01 | 220,665^ |
| Moderately expressed | |||||
| let-7i | 7.15 ± .30 | 6.69 ± .71 | −0.34 | −0.99 | 560 |
| miR-200a | 6.68 ± .55 | 5.95 ± .88 | −0.09 | −0.75 | 1^,345 |
| let-7e | 6.66 ± .33 | 6.27 ± .85 | −0.34 | −1.01 | 563 |
| let-7g | 6.12 ± .37 | 5.43 ±1.32 | −0.34 | −0.99 | 561 |
| miR-425 | 4.13 ± .26 | 3.91 ± .56 | −0.08 | −0.88 | 578,802^ |
| miR-185 | 4.40 ± .40 | 3.76 ± .79 | −0.27 | −0.59 | 54,618^ |
a
Toray 3-D Gene data is expressed as the mean of at least four trials and standard deviation.
b
Total context score as computed by Targetscan.org. The total context score represents the sum of the contribution of six features: site-type contribution, 3' pairing contribution, local AU contribution, position contribution, target site abundance contribution, and seed-pairing stability contribution.
c
mirSRV score as computed by microRNA.org.
d
Position of the first base of a seed sequence within the 3’UTR. ^ indicates the major seed sequence(s) contributing to the mirSRV score.