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. 2014 Aug 29;14:232. doi: 10.1186/s12870-014-0232-4

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© Pflieger et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Screening of P. vulgaris cultivars for susceptibility to Bean pod mottle virus (BPMV). (A) Mock inoculated plants (top panel) and BPMV-0 inoculated plants (bottom panel) were photographed under natural light at 28 days post-inoculation (dpi). For BPMV-0, mechanical inoculation was made by rubbing of infected leaf sap. (B) Semi-quantitative RT-PCR of BPMV RNA1 and RNA2 in mock- and BPMV-0 treated plants. Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 21 dpi from the third trifoliate leaf of three plants for Black Valentine, BAT93 and G19833 and from the second trifoliate leaf for JaloEEP558.