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. 2014 Aug 29;14:232. doi: 10.1186/s12870-014-0232-4

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© Pflieger et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Silencing of PDS in P. vulgaris cv. Black Valentine using the VIGS vector BPMV-GmPDS-327 bp. (A-B) Plants inoculated with mock buffer (left panel), BPMV-0 (middle panel), and BPMV-GmPDS-327 bp (right panel) were photographed under natural light at 28 days post-inoculation (dpi). (B) Trifoliate systemic leaves. (C) Semi-quantitative RT-PCR of PvPDS, BPMV RNA1 and RNA2 in systemic leaves of plants inoculated with mock, BPMV-0, and BPMV-GmPDS-327 bp. Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 21 dpi from the third trifoliate leaf of three different plants of Black Valentine.