Table 3.
PCR primers used for construction of the BPMV VIGS vectors and RT-PCR analyses
| Name of primer | DNA sequence of primer (5′-3′) | Size of PCR product (bp) |
|---|---|---|
| PvaPDS-F1 | cgggatccAGCAGAAGTCCCCTTCTGAG | 391 |
| PvaPDS-R1 | cgggatccCTTGTGCACACAGCTTCC | |
| PvaPDS-262fwd | cgggatccAGAATGGATTTCACGTAGT | 262 |
| PvaPDS-262rev | cgggatccAACAGCACCTTCCATTGAAGCT | |
| PvaPDS132-rev | cgggatccGGTGTTTTAACAACATGGTAC | 132a |
| PvaPDS52-fwd | GATCGCTTTGCTTTGGTCTGCAGAAATTTCATCAGGAAAGAGTTTGGCAAGCTCAGT | - |
| PvaPDS52-rev | GATCACTGAGCTTGCCAAACTCTTTCCTGATGAAATTTCTGCAGACCAAAGCAAAGC | - |
| RNA1-fwd | CAAGCCCAAAGTGCTGAAGT | 160 |
| RNA1-rev | GCAAATCCAACTGCCATTCT | |
| RNA2-fwd | ATACCCCTAATGGCACAGGA | 269 |
| RNA2-rev | GGAAATGTAACCACCCGAAT | |
| RNA2-BamH1-fwd | TGACAATCCCAAACAGTCTACAG | 234 |
| RNA2-BamH1-rev | AGCATACTCAACGAGAGGGTCA | |
| RNA2-GFP-fwd | TGAGGTTCAGGCTCAGATGGA | 863 |
| RNA2-GFP-rev | GGCCTGGATTTGATTCTACA | |
| PvPDS-fwd | ACATCTTCTTTGGGGCTTACC | 200 |
| PvPDS-rev | CAGCATCTCGTTGTTCCTCA | |
| PvUBI-fwd | CAGCTGGAGGATGGAAGG | 200 |
| PvUBI-rev | TCCGAACTCTCCACCTCAAGA |
ain combination with PvaPDS-262fwd.