Table 2.
Real-time RT-PCR analysis of suspected key components of the response to chronic SIV-infection
| Gene | Microarray | RT-PCR | ||
|---|---|---|---|---|
| Acute 1 | Chronic 1 | Acute | Chronic | |
| Cav-1 | 1 | 4.82 | 1 | 4 |
| CCL3 | 1 | 0.7 | 1 | 1 |
| CCR5 | 1 | 1.4 | n. d.2 | >20 |
| CD3Z | 1 | 11.68 | 1 | 13 |
| CD4 | 1 | 0.12 | 1.8 | 0.06 |
| CD44 | 1 | 2.69 | 1.35 | 11 |
| Fut-7 | 1 | 0.12 | 1.25 | 0.095 |
| IL2-RA | 1 | 0.59 | 1.25 | 0.29 |
1RNA from acutely or chronically SIVmac251-infected or mock-infected C8166 T cells was used for the experiments. Values represent the ratios between signals (average fold change) derived from infected and mock-infected cells and are derived from at least 2 independent experiments.
2not detectable.