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. Author manuscript; available in PMC: 2015 Jan 11.
Published in final edited form as: Science. 2014 Jul 11;345(6193):220–222. doi: 10.1126/science.1254522

Fig. 2. MTSES specifically inhibits the function of MurJA29C.

Fig. 2

(A) Structural model of MurJ (8). Sensitivity to MTSES is limited to specific residues within the MurJ cavity: residues 29 (green) in transmembrane domain (TMD) 1, 49 (red) in TMD 2 , and 263 (orange), 269 (blue) and 273 (magenta) in TMD 8. (B) Effect of MTSES on the growth of haploid cells producing MurJWT (left) or MurJA29C in glucose M63 medium. Lysis of MurJA29C cells is suppressed by the presence of a wild-type murJ allele (right). Arrows indicate time of MTSES addition; filled marker, no MTSES; empty marker, MTSES treated. Data represent mean ± SD; N = 3. See fig. S5 for MTSES sensitivity of other variants.