Table I.
The effect of different concentrations of MEM vitamins on the in vitro maturation and development of sheep oocytes
| Treatments | Oocytes (n) | Cleavage (%) | Morula (%) * | Blastocyst (%) * |
|---|---|---|---|---|
| SOF | 246 | 58.58±6.77a (144) | 36.74±5.25a (53) | 19.50±1.16ab (28) |
| SOF + 0.5×MEM | 250 | 60.03±4.86a (150) | 34.88±3.05a (52) | 21.62±1.02a (32) |
| SOF + 1 × MEM | 251 | 67.07±5.71a (168) | 39.08±5.02a (65) | 22.33±1.11a (37) |
| SOF + 1.5 × MEM | 254 | 70.44±4.19a (179) | 33.21±5.23a (59) | 15.56±2.0b (28) |
Note: Data expressed as mean±SEM of six replicates. SOFM= synthetic oviduct fluid medium (control); SOF+0.5×MEM=SOF maturation medium supplemented with 0.5×MEM vitamins; SOF+1×MEM=SOF maturation medium supplemented with 1×MEM vitamins; SOF+1.5×MEM=SOF maturation medium supplemented with 1.5×MEM vitamins.
*
Morula and blastocysts per cleaved oocytes.
a,b
Different letters indicate statistical difference within each column (p<0.05).