Figure 2.

Morphological examination of epidermis. TPF image of NADH autofluorescence acquired from the epidermis of a healthy male volunteer at 25 μm depth from skin surface (a), 40 μm depth from skin surface (b), and 55 μm depth from skin surface (c). The images are approximately corresponding to granular layer (a), spinous layer (b), and basal layer (c). Laser power, measured after the objective, is indicated in the down-left corner of each image. Excitation wavelength: 740 nm. Field of view: 200 μm. Scale bar: 20 μm.