Table 1.
HRM primers and amplicon information.
| Gene name | Primer sequences 5′-3′ | GenBank | Number of CpG-sites/length |
|---|---|---|---|
| Accession number | of amplified fragment | ||
| MGMT | Fw GCGTTTCGGATATGTTGGGATAGT Rv AACGACCCAAACACTCACCAAA |
X61657 | 15/110 |
|
| |||
| MLH1 | Fw TTTTTTTAGGAGTGAAGGAGG Rv AACRCCACTACRAAACTAAA |
AY217549 | 13/123 |
|
| |||
| APC | Fw AAGTAGTTGTGTAATTCGTTGGAT Rv CACCTCCATTCTATCTCCAATA |
NT_034772 | 11/149 |
|
| |||
| p16 | Fw GGAGTTTTCGGTTGATTGGTTGGTT Rv AACAACGCCCGCACCTCCTCTA |
AF527803 | 5/69 |
|
| |||
| MINT1 | Fw GGGGTTGAGGTTTTTTGTTAG Rv AATCCCTCTCCCCTCTAAACTT |
AF135501 | 7/137 |
|
| |||
| MINT31 | Fw GGGTGATGGTTTTAGTAAAGTGAG Rv AAAAACACTTCCCCAACATCTAC |
AF135531 | 10/164 |
|
| |||
| RUNX3 | Fw TTTTTAGAGAATGAGGGATTTTTGT Rv CCCTAATCCCTTAAATCTAATACCC |
NC_000001 | 7/115 |