Figure 2.

sPLA₂X secretion and relative expression of cPLAα in PBMC of asthmatics (a) and healthy subjects (b) in response to rDer p1. PBMC (2 × 10⁶) were stimulated with indicated doses of rDer p1 for 24 hours. Control represents cells treated with the vehicle. The bar graph shows the densitometry results for cPLA₂ (immunoblotting results) and ELISA results for sPLA₂X secretion. Data are presented as the fold change compared with the vehicle-treated cells. Data represent the mean ± SE from at least six independent experiments. ∗P < 0.05 shows comparison between relative protein expression of cPLA₂ and secretion of sPLA₂X.