Table 1.

Details of primers used for PCR amplification of GsSGT ORF, heterologous expression, and real-time expression profiling.

Primer details	Primer's name	Nucleotide sequence of primer (5′-3′)	Length (mer)	Tm (°C)	GC content (%)	Size of the amplicon
Primers used for partial clone, full length cDNA and heterologous expression
Degenerate	WsSGTF2	TGGCTACATCTGGACTCCTCAT	22	57.3	50.0	370 bp
	WsSGTR2	TGGTTCATCACGGTGGTGCT	20	59.4	55.0
3′RACE	GymGTF1	GGCACTGGAGAATACAGGACAGAG	24	58.8	54.2	684 bp
	GymGTF2	CCTCGTTGATTGCCCTCATGACTG	24	59.9	54.2
5′RACE	GymGTR1	GGTTTAGGCCCCTTCTGTATCCAC	24	59.2	54.2	1.7 kb
	GymGTR2	CCAACAACATCAACCAGAGGGCC	23	60.6	56.5
	GymURD01	AGAGACTCGATGATGGCCTTGAGC	24	60.5	54.2
	GymURD02	AATCACTGAAGTTAGCATGAGTAGCCAGCC	30	61.6	46.7
	GymURD03	ACTCCTGTAATTTCTTAGCCATAGCC	26	56.5	42.3
Complete ORF	2.3GymFLF1	TTGGATTAACATATTTGAGCATCCAGGC	28	57.6	39.3	2.1 kb
	2.3GymFLF2	TTGAGCATCCAGGCTTCTCTTGCATGG	27	62.8	51.9
	2.3GymFLR1	ATTCTGTAATTCCTTGTTCCGTTGTAAACC	30	57.5	36.7
pET-28a expression	PTGGTF0N	CATATGGATTCTGATGGGACTGGC	24	57.4	50.0	2.1 kb
	PTGGTR0B	GGATCCCTAAGAACAACAAGGCAGG	25	59.4	52.0
Primers used for semiquantitative and real-time PCR expression studies
RT-PCR	Actin3F	TTGCCGAGATAATGGCCTAC	20	54.6	50.0	250 bp
	Actin3R	TACCGCGACTTCGATCTTTT	20	54.0	45.0
	SGTF2	TGGCTACATCTGGACTCCTCAT	22	57.3	50.0	370 bp
	SGTR2	TGGTTCATCACGGTGGTGCT	20	59.4	55.0
Real-time PCR	PTGymGTF2	CCTCGTTGATTGCCCTCATGACTG	24	59.9	54.2	350 bp
	PT1.8GymFL1	GGATCCCTAAGAACAACAAGGCAGGCTG	28	62.7	53.6

∗Restriction sites for pET-28a expression are highlighted in bold and underlined.