Table 1.
Bacterial strains and plasmids.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strain | ||
| ExPEC strain PPECC42 | Wild-type (WT), porcine origin, CmS | Lab stock |
| PPECC42ΔtolC | Mutant with a 158-bp fragment deleted from the whole ORF of the tolC gene in PPECC42, CmS | This study |
| Cm-tolC | PPECC42ΔtolC strain containing plasmid pHSG-tolC, CmR | This study |
| χ7213 | Thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP4 2-Tc::Mu[λpir] KmR | [22] |
| χ7213-ΔtolC | χ7213 containing suicide vector pRE112ΔtolC | This study |
| DH5α | F−, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk −, mk +), phoA, supE44, λ −, thi-1, gyrA96, relA1 | Takara Bio |
| Plasmid | ||
| pRE112 | oriT oriV Δasd CmR SacB, suicide vector | [22] |
| pRE ΔtolC | pRE112-inserted disrupted tolC gene in Kpn I and Sac I sites | This study |
| pHSG396 | ori lacZ CmR | Takara Bio |
| pHSG-tolC | CmR | This study |