Figure 1.

Flow cytometry analysis of TFH cells. PBMCs from MCD patients' pre- and posthormone drugs treatment as well as HC were stained with anti-CD4, anti-CD3, anti-CXCR5, anti-ICOS, and anti-PD-1. The cells were gated initially on living lymphocytes and then on CD3⁺CD4⁺ T cells. Subsequently, the frequency of CD4⁺CXCR5⁺, CD4⁺CXCR5⁺ICOS⁺, CD4⁺CXCR5⁺PD-1⁺, and CD4⁺CXCR5⁺PD-1⁺ICOS⁺ TFH cells was analyzed by flow cytometry. (a) Flow cytometry analysis and (b) quantitative analysis. Data shown are representative dot plug or expressed as the mean % of different subsets of TFH cells in total CD3⁺CD4⁺ T-cells individual subjects from two separate experiments. The horizontal lines represent the median values.