Figure 6.

Flow cytometry analysis of B cells. The relationship of the percentages of different subsets of T follicular helper cells (TFH) with B cells in MCD patients. PBMCs from MCD patients and HC were stained with anti-CD19, anti-CD38, anti-CD95, and anti-IgD. The cells were gated initially on living lymphocytes and then on CD19⁺ B cells. Subsequently, the frequency of CD19⁺IgD⁺CD27⁻ B cells, CD19⁺IgD⁺CD27⁺ B cells, CD19⁺IgD⁻CD27⁺ B cells, CD19⁺IgD⁻CD27⁻ B cells, CD19⁺CD86⁺ B cells, and CD19⁺CD95⁺ B cells was analyzed by flow cytometry. (a) Flow cytometry analysis, (b) quantitative analysis, and (c) the percentages of different subsets of T follicular helper (TFH) and B cells. Data shown are representative dot plug or expressed as the mean % of different subsets of B cells. The horizontal lines represent the median values.